non-specific control sirna  (SignaGen)

Journal: Alzheimer's Research & Therapy

Article Title: tRF Ala-AGC-3-M8 attenuates neuroinflammation and neuronal damage in Alzheimer’s disease via the EphA7-ERK 1/2 -p70S6K signaling pathway

doi: 10.1186/s13195-025-01734-6

Figure Lengend Snippet: Effects of tRF Ala−AGC−3−M8 on ERK 1/2 -p70S6K pathway in BV-2 cells. (A, I) qRT-PCR analysis of tRF Ala−AGC−3−M8 and EphA7 in BV-2 cells after transfection with tRF Ala−AGC−3−M8 -mimic or EphA7-siRNA (n = 6, biologically independent samples). (B-D, J-L) Western blot analysis of EphA7 and phosphorylation of ERK 1/2 and p70S6K (T421/S424) in BV-2 cells (n = 6, biologically independent samples). (E, M) Representative light microscopy images of BV-2 cells morphological features; Scale bar: 75μm (overview), 20μm (insert). (F-H, N-P) Immunofluorescence localization an mean fluorescence intensity of iNOS in BV-2 cells ( n = 6, biologically independent samples); Scale bar: 75μm (overview), 20μm (insert); Statistical significance was assessed using Student’s test (A and I) and one-way ANOVA (D, L, G and O) (P values are indicated). All data are presented as the means ± SEM. Each data point represents the average of 3 technical replicates

Article Snippet: EphA7-targeting siRNA (EphA7-siRNA) and non-specific control siRNA (NC-siRNA), as well as chemically synthesized double-stranded tRF Ala-AGC-3-M8 mimic (tRF Ala-AGC-3-M8 -mimic) and non-specific control mimics (NC-mimic), were purchased from RIBOBIO Biotechnology Co., Ltd., China.

Techniques: Quantitative RT-PCR, Transfection, Western Blot, Phospho-proteomics, Light Microscopy, Immunofluorescence, Fluorescence

Journal: Alzheimer's Research & Therapy

Article Title: tRF Ala-AGC-3-M8 attenuates neuroinflammation and neuronal damage in Alzheimer’s disease via the EphA7-ERK 1/2 -p70S6K signaling pathway

doi: 10.1186/s13195-025-01734-6

Figure Lengend Snippet: Effects of tRF Ala−AGC−3−M8 on ERK 1/2 -p70S6K pathway in HT22 cells. (A) qRT-PCR analysis of tRF Ala−AGC−3−M8 and EphA7 in HT22 cells after transfected with tRF Ala−AGC−3−M8 -mimic or EphA7-siRNA ( n = 6, biologically independent samples). (B-C) Western blot analysis of EphA7 expression and phosphorylation levels of ERK 1/2 and p70S6K (T421/S424) ( n = 6, biologically independent samples). (D) Representative image of HT22 morphological features; Scale bar: 75μm (overview), 20μm (insert). (E, F, G) Immunofluorescence localization and mean fluorescence intensity of p-tau (Red) in HT22 cells ( n = 6, biologically independent samples), p-tau was labelled with solid white line in zoomed-in immunofluorescence image; Scale bar: 75μm (overview), 20μm (insert); Statistical significance was assessed using Student’s test (A) and one-way ANOVA (C and F) (P values are indicated). All data are presented as the means ± SEM. Each data point represents the average of 3 technical replicates

Article Snippet: EphA7-targeting siRNA (EphA7-siRNA) and non-specific control siRNA (NC-siRNA), as well as chemically synthesized double-stranded tRF Ala-AGC-3-M8 mimic (tRF Ala-AGC-3-M8 -mimic) and non-specific control mimics (NC-mimic), were purchased from RIBOBIO Biotechnology Co., Ltd., China.

Techniques: Quantitative RT-PCR, Transfection, Western Blot, Expressing, Phospho-proteomics, Immunofluorescence, Fluorescence

Journal: Scientific Reports

Article Title: IQGAP3 activates Hedgehog signaling to confer stemness and metastasis via up-regulating GLI1 in lung cancer

doi: 10.1038/s41598-024-82793-x

Figure Lengend Snippet: IQGAP3 promoted the migration, invasion and stemness potential of lung cancer. ( A ) Down-regulation of IQGAP3 in H1299 and A549 cells after IQGAP3 siRNA transfection. original blots/gels are presented in Supplementary Fig. 3 .( B-C ) Representative images and quantitative data of wound healing assays in H1299 and A549 cells after IQGAP3 down-regulation. Scale bar = 200 μm. ( D-F ) Representative images and quantitative data of Transwell migratory and invasive assays in H1299 and A549 cells after IQGAP3 down-regulation. Scale bar = 50 μm. ( G-H ) Sphere formation ability of lung cancer cells via sphere formation assays after silencing IQGAP3. Scale bar = 100 μm. Data are shown as mean ± SD from experiments in triplicate. ** P < 0.01, *** P < 0.001, ns not significant.

Article Snippet: A549 and H1299 cells were cultured in six-well plates and transfected with either IQGAP3 siRNA or a non-specific control siRNA using GenMute Reagent (SignaGen), following the manufacturer’s protocols.

Techniques: Migration, Transfection

Journal: Scientific Reports

Article Title: IQGAP3 activates Hedgehog signaling to confer stemness and metastasis via up-regulating GLI1 in lung cancer

doi: 10.1038/s41598-024-82793-x

Figure Lengend Snippet: Activation of Hedgehog pathway rescues IQGAP3 knockdown-induced inhibition of lung cancer migration, invasion and stemness. ( A-B ) Representative images and quantified data from wound healing assays performed in H1299 cells after IQGAP3 siRNA and SAG treatment. Scale bar = 200 μm. ( C-D ) Representative images and quantified data illustrating Transwell migratory assays conducted in H1299 and A549 cells following IQGAP3 siRNA and SAG treatment. Scale bar = 50 μm. ( E-F ) Assessment of invasion ability in H1299 and A549 cells using Transwell invasive assays after IQGAP3 down-regulation and SAG addition. Scale bar = 50 μm. ( G-H ) Sphere formation capacity of lung cancer cells evaluated through sphere formation assays after silencing IQGAP3 and SAG treatment. Scale bar = 100 μm. Data represent mean ± SD from experiments conducted in triplicate. ** P < 0.01, *** P < 0.001, ns not significant.

Article Snippet: A549 and H1299 cells were cultured in six-well plates and transfected with either IQGAP3 siRNA or a non-specific control siRNA using GenMute Reagent (SignaGen), following the manufacturer’s protocols.

Techniques: Activation Assay, Knockdown, Inhibition, Migration